Kinetic Parameters from Detection Probability in Single Molecule Force Spectroscopy
نویسندگان
چکیده
منابع مشابه
Kinetic parameters from detection probability in single molecule force spectroscopy.
The detection probability of rupture events in AFM force spectroscopy measurements presents a viable alternative to standard methods for extracting kinetic parameters of dissociation. The detection probability has a maximum as a function of the probe velocity where (1) the probability to form a molecular bond is independent of the probe velocity and (2) the detection of rupture events is limite...
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In typical force spectroscopy experiments, a small biomolecule is attached to a soft polymer linker that is pulled with a relatively large bead or cantilever. At constant force, the total extension stochastically changes between two (or more) values, indicating that the biomolecule undergoes transitions between two (or several) conformational states. In this paper, we consider the influence of ...
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The deflection of a cantilever is measured using a laser beam and position-sensing detector to obtain force and displacement for both imaging and force spectroscopy. In force spectroscopy, the cantilever is attached to one end of the protein or DNA sample while the other end is immobilized on the surface. The force and position of the tip are measured as the surface is moved away from the tip, ...
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The direct measurement of forces between individual receptor-ligand complexes is an emerging new field. With the atomic force microscope (AFM) it has been possible to probe the forces between a range of biological molecules, including complementary oligo-nucleotides, proteins and carbohydrate interactions. To date, the force required to rupture these single ligandreceptor interactions is common...
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ژورنال
عنوان ژورنال: Langmuir
سال: 2010
ISSN: 0743-7463,1520-5827
DOI: 10.1021/la101269q